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Karolinska universitetssjukhuset
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Elisabeth Nyström Team

Understanding mucus from molecule to man

Mucus (green) separates luminal bacteria (red) from the underlying host epithelium (blue/grey).

About

Our research focuses on the function and regulation of the intestinal mucus layer. As the interface between the gut epithelium and the vast microbial community, the mucus barrier plays a central role in host–microbiota interactions and tissue protection.

Our aim is to understand how mucus and mucus-producing goblet cells dynamically shape host–microbiota interactions as an innate immune interface in health and disease. For this, we take an interdisciplinary approach, combining cell and protein omics, targeted in vivo cell manipulation, specialized mucus analysis pipelines, and protein biochemistry. We are particularly interested in how post-translational protein modifications regulate these processes and to develop ex vivo live tissue imaging to study host–microbe interactions in real time.

Team leader

Elisabeth Nyström is a principal investigator at Karolinska Institutet, where she leads a research program focused on mucus biology and mucosal immunology. She obtained her PhD in Medical Biochemistry and Cell Biology at the University of Gothenburg in the Mucin Biology Group, and completed her postdoctoral training in the Becker-Pauly laboratory at Christian-Albrechts-University of Kiel, where she developed a strong interest in mucus biology and the regulatory role of post-translational modifications.

elisabeth.nystrom@ki.se

Team members

Dinesh Nirmalan, Research assistant, MSc, dinesh.nirmalan@ki.se

Selected publication

Cryo-EM structure of CLCA1 identifies CLCA1 as a founding member of a novel metzincin family. Nyström E, van der Post S, Barrett DB, Raba G, Pelaseyed T, Oltean M, et al. bioRxiv 2025; doi:10.1101/2025.10.18.683246

Neonatal microbiota colonization primes maturation of goblet cell-mediated protection in the pre-weaning colon. Johansson Å, Venkita Subramani M, Yilmaz B, Nyström EEL, Layunta E, Arike L, et al. J Exp Med 2025;222(8)

Proteolytic processing of galectin-3 by meprin metalloproteases is crucial for host-microbiome homeostasis. Bülck C, Nyström EEL, Koudelka T, Mannbar-Frahm M, Andresen G, Radhouani M, et al. Sci Adv 2023;9(13):eadf4055

An intercrypt subpopulation of goblet cells is essential for colonic mucus barrier function. Nyström EEL, Martinez-Abad B, Arike L, Birchenough GMH, Nonnecke EB, Castillo PA, et al. Science 2021;372(6539)

Calcium-activated chloride channel regulator 1 (CLCA1) forms non-covalent oligomers in colonic mucus and has mucin 2-processing properties. Nyström EEL, Arike L, Ehrencrona E, Hansson GC, Johansson MEV. J Biol Chem 2019;294(45):17075-17089

Full list of publications: https://scholar.google.com/citations?user=FS8L1g0AAAAJ

About CMM

The Center for Molecular Medicine (CMM) is a foundation instituted by the Stockholm County Council (Region Stockholm). CMM is at the heart of a close partnership with the Karolinska University Hospital and Karolinska Institutet, fueling advancements in biomedical and clinical research.

Contact

Center for Molecular Medicine Foundation, org. nr. 815201-3689

Karolinska University Hospital L8:05

Visionsgatan 18

171 76 Stockholm, Sweden

communication@cmm.se

CMM
Karolinska institutet
Karolinska universitetssjukhuset